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This experimental process demonstrates the potential of advancing the technology of lignocellulosic-based biofuels. Maximized bio-oil yields and gasoline range aromatics were obtained from the pyrolysis of switchgrass biomass in a medium-scale fixed bed reactor (48.2 L). The reaction’s final temperature was set at 520ºC while the carrier gas flow rate was varied at (50 L min-1, 75 L min-1 and 100 L min-1) both without and with the use of the ZSM-5 catalyst. Bio-oil yields of 18.2%, 26.9%, and 34.1% were generated without catalyst. Using the ZSM-5 catalyst, bio-oil yields of 20.2%, 41.5%, and 47.7% were generated. At 75 L min-1, 9.6% gasoline range organics (GROs) were detected without the catalyst and 12.4% aromatics were detected in the experiment with ZSM-5 catalyst. At 100 L min-1, 10.5%, and 13.7% of aromatics were detected without and with ZSM-5 catalyst respectively. At 75 L min-1, 14.5% of oxygen content recorded without catalyst, and 5.6% with ZSM-5 catalyst. At 100 L min-1, oxygen content was 10.4% without catalyst, and 8.6 % with ZSM-5 catalyst. The effects of carrier gas flow rate variations and a ZSM-5 catalyst on bio-oil yields and quality were experimentally demonstrated using a single-step thermochemical conversion process. This is a major development towards improving U.S energy security and achieving global CO2 emissions mitigation targets.